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    Phosphate Buffered Saline, PBS (pH 7.4, Sterile-Filtered)

    Phosphate Buffered Saline, PBS (pH 7.4, Sterile-Filtered)

    Phosphate Buffered Saline (PBS) is a versatile buffer solution widely used in biological research due to its isotonic and non-toxic nature to cells. With a pH range of 7.0 to 7.4, PBS mimics the osmolarity and ion concentrations of the human body, making it ideal for a variety of applications including protein purification, as wash buffer in Western blot and immunoassays, sample dilution, and as a transport medium for tissue samples as well as RNA viruses.

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    Tris-Glycine-SDS Running Buffer (10X)

    Tris-Glycine-SDS Running Buffer (10X)

    The Tris-Glycine-SDS Running Buffer is the most commonly used buffer for the separation/resolution of proteins in their denatured state during SDS-PAGE. It serves as both the anode and cathode buffer during gel electrophoresis and helps to regulate the pH of the electrophoresis system. When diluted to 1X, this buffer regulates pH levels at a pH of about 8.6, which provides reproducible separation of a wide range of proteins into well-resolved bands, depending upon the gel percentage used.

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    Transfer Buffer (10X, Electro Blotting)

    Transfer Buffer (10X, Electro Blotting)

    The 10X Transfer Buffer-Electro Blotting is the most common buffer used in the wet transfer system for the electro transfer of proteins. The buffer facilitates protein transfer from SDS-PAGE gels to nitrocellulose or PVDF membranes. Ready-to-use solution once diluted to 1X working concentration; Methanol can be added to the final concentration of 10-20% (v/v) as per user protocol. This buffer prevents the gel from swelling during transfer and enhances efficiency of low abundancy proteins and protein binding to nitrocellulose membrane. This buffer is also used as a running buffer for native protein gel electrophoresis.

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Researcher working with hybridization buffers in a molecular biology lab
  1. Home
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  4. Hybridization Buffers

Hybridization Buffers

Hybridization buffer is designed to create the optimal environment for nucleic acid probe binding in applications like in situ hybridization and nucleic acid hybridization. Its precise formulation promotes high-specificity hybridization while reducing background noise, ensuring accurate detection of target DNA or RNA sequences.

  • Maintains optimal pH and ionic strength to support specific probe-target binding
  • Reduces non-specific interactions for clear, high-sensitivity results
  • Compatible with DNA and RNA workflows including blots, microarrays, and ISH

Whether you're mapping gene expression or detecting viral RNA, our hybridization buffers provide the reliability and sensitivity your nucleic acid assays require.

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Hybridization Buffers

Showing 1–5 of 5 products

Saline-Sodium Phosphate-EDTA Buffer, SSPE (20X, RNase Free)

Saline-Sodium Phosphate-EDTA Buffer, SSPE (20X, RNase Free)

From: $98.00

Saline-Sodium Citrate (20X)

Saline-Sodium Citrate (20X)

From: $82.00

Saline-Tris-EDTA Buffer, STE (Nuclease Free, pH 8.0)

Saline-Tris-EDTA Buffer, STE (Nuclease Free, pH 8.0)

From: $90.00

Denhardt’s Solution (50X)

Denhardt’s Solution (50X)

From: $94.00

Denhardt’s Solution (100X)

Denhardt’s Solution (100X)

From: $115.00

FAQs

A hybridization buffer facilitates the binding of complementary nucleic acid probes to their target DNA or RNA during techniques such as in situ hybridization and blotting assays.

These buffers typically contain salts (e.g., sodium chloride or phosphate), detergents (e.g., SDS), and sometimes formamide or dextran sulfate to control stringency and reduce non-specific binding.

By controlling ionic strength and including blocking agents, hybridization buffers minimize non-specific interactions, enhancing the signal-to-noise ratio and improving detection sensitivity.