Lysis buffers are a critical reagent designed to break open cells and efficiently release proteins, nucleic acids, and other intracellular components for downstream analysis. The detergent and salt composition is optimized to ensure high-yield extraction while preserving the integrity of your target molecules.
Enables efficient, reproducible cell lysis across a variety of sample types
Protects proteins and nucleic acids from degradation during extraction
Compatible with workflows such as protein purification, DNA/RNA isolation, and cell-based assays
Whether you're working on high-throughput screening, genomic extraction, or proteomic analysis, our lysis buffers deliver the consistent performance needed to drive reliable results.
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Frequently Asked Questions
What are the main components of a lysis buffer?
Typical lysis buffers contain detergents (e.g., Triton X-100, SDS), salts (e.g., NaCl), buffering agents (e.g., Tris), and sometimes enzyme inhibitors (e.g., DNase or protease inhibitors) to enhance extraction efficiency and protect target molecules.
How do I choose the right lysis buffer for my experiment?
The ideal lysis buffer depends on the cell type (e.g., bacterial, mammalian) and your target molecule. Non-denaturing buffers are ideal for preserving protein activity, while denaturing buffers are used in techniques like electrophoresis and western blotting. DNA and RNA extraction buffers vary depending on the protocol and purity requirements.
Does lysis buffer affect downstream applications like PCR or western blot?
It can, residual detergents or salts may interfere if not properly removed. Use a compatible lysis buffer or follow with purification steps to avoid inhibition.
