NP-40 lysis buffer is a non-ionic detergent-based solution designed for gentle cell lysis while preserving protein complexes and nuclear structure. It’s ideal for applications where complete disruption isn’t necessary, allowing selective solubilization of cytoplasmic and membrane proteins.
Provides controlled lysis of cell and organelle membranes without full nuclear disruption
Preserves protein-protein interactions for immunoprecipitation and co-IP workflows
Ideal for preparing lysates for ELISA, western blotting, and enzyme assays
Whether you're isolating cytoplasmic proteins, studying membrane dynamics, or capturing native protein complexes, our NP-40 lysis buffer offers a precise balance between disruption and preservation.
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Frequently Asked Questions
What is NP-40 lysis buffer used for?
NP-40 buffer is used for mild cell lysis, allowing extraction of cytoplasmic and some membrane-associated proteins while preserving nuclear and chromatin structure.
How does NP-40 compare to RIPA buffer?
NP-40 is gentler than RIPA, making it better suited for extracting proteins while preserving interactions. RIPA is harsher and suited for complete cell lysis and membrane solubilization.
What downstream applications are compatible with NP-40 lysates?
Lysates prepared with NP-40 are compatible with ELISA, western blotting, enzyme activity assays, and protein quantification methods.
