Tris-Glycine-SDS Running Buffer (10X)
CAT#: BP-150
- Useful in biochemistry and molecular biology techniques
- Effective as a running buffer for SDS polyacrylamide gel electrophoresis
- Ready-to-use solution tested for pH
Available sizes:
Product Description
The Tris-Glycine-SDS Running Buffer is the most commonly used buffer for the separation/resolution of proteins in their denatured state during SDS-PAGE. It serves as both the anode and cathode buffer during gel electrophoresis and helps to regulate the pH of the electrophoresis system. When diluted to 1X, this buffer regulates pH levels at a pH of about 8.6, which provides reproducible separation of a wide range of proteins into well-resolved bands, depending upon the gel percentage used.
Specifications
| Method: | Filtered through 1-micron filter. |
|---|---|
| Storage: | Room Temp. |
| pH: | 8.50 ± 0.20 (1X) |
| QC Testing: | Appearance, pH |
| Notes: | To make 1X, dilute 10-fold with deionized water. |
| Component Name | CAS | Molecular Weight | Concentration |
|---|---|---|---|
| Tris-Base | 77-86-1 | 121.14 | 250 mM |
| Glycine | 56-40-6 | 75.07 | 1.92 M |
| Sodium Dodecyl Sulfate (SDS) [20% Solution] | 151-21-3 | 288.38 | 1% |
| Water | 7732-18-5 | 18.02 | Rest |
Product Citations
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