Tricine buffer (N-tris(Hydroxymethyl)methylglycine) is a versatile Good’s buffer from the Tris family, widely used in biochemistry and molecular biology. Known for its strong buffering capacity in electrophoresis and chromatography, Tricine is the preferred SDS-PAGE buffer for separating low molecular weight polypeptides (1–100 kDa). It also plays a key role in capillary zone electrophoresis, HPLC, ion exchange chromatography, and cell resuspension applications.
Optimized for low molecular weight protein separation in SDS-PAGE
High stability & solubility ensures reproducible experimental results
Compatible with electrophoresis & chromatography for accurate molecular analysis
Whether you're working with protein separation, high-resolution chromatography, or molecular biology workflows, Tricine buffer offers exceptional stability and precision for high-quality results.
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Frequently Asked Questions
Why is Tricine buffer preferred for low molecular weight protein separation in SDS-PAGE?
Tricine has a lower ion mobility than glycine, providing better resolution for small polypeptides (1–100 kDa) in SDS-PAGE compared to traditional Tris-glycine buffers.
How does Tricine buffer compare to Tris-glycine in electrophoresis?
While Tris-glycine is commonly used for standard protein electrophoresis, Tricine is superior for separating smaller proteins and peptides due to its enhanced resolving power.
Can Tricine buffer be used in chromatography applications?
Yes! Tricine is frequently used in capillary zone electrophoresis, high-performance liquid chromatography (HPLC), and ion exchange chromatography due to its buffering efficiency and compatibility with protein and peptide analysis.
