Achieve precise pH control in your experiments with MES buffer, a trusted Good’s buffer for biological and biochemical research. With a pKa of 6.1 at 25°C, MES buffer is ideal for maintaining a stable, slightly acidic pH (5.5 to 6.7), ensuring reproducibility and accuracy in protein purification, cell culture, and enzyme assays.
Highly soluble for easy preparation in aqueous solutions
Chemically stable to prevent degradation and pH fluctuations
Minimal metal ion binding ensures compatibility with sensitive biochemical reactions
If you're optimizing buffer conditions for protein stability or conducting high-precision assays, MES buffer provides the stability and reliability needed for consistent experimental outcomes.
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Frequently Asked Questions
What makes MES buffer ideal for biological experiments?
MES buffer maintains a stable, slightly acidic pH (5.5–6.7), making it suitable for enzyme assays, protein purification, and cell culture. Its low metal ion binding reduces interference in sensitive biochemical reactions.
How do I prepare MES buffer for my experiment?
Dissolve the appropriate amount of MES powder in deionized water, adjust the pH with NaOH or HCl as needed, and filter-sterilize if required. The exact concentration will depend on your experimental setup.
Is MES buffer compatible with cell culture and protein purification?
Yes! MES is widely used in cell culture and protein purification due to its stability, low reactivity with biomolecules, and compatibility with various biological systems.
