TAPS buffer (N-[Tris(hydroxymethyl)methyl]-3-aminopropanesulfonic acid) is a reliable Good’s buffer for molecular biology, providing stable pH control in moderately alkaline conditions. With a pKa of approximately 8.44, TAPS is effective in the pH range of 7.7 to 9.1, making it an excellent choice for capillary electrophoresis, chromatography, enzyme assays, and cell culture media.
Stable alkaline pH for DNA analysis and biochemical assays
Highly soluble & chemically stable for consistent results
Low interference with biomolecules ensures accuracy in sensitive experiments
Whether you’re working with DNA separation, dye chromatography, or enzymatic reactions, TAPS buffer provides precision and reliability for high-performance molecular biology applications.
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Frequently Asked Questions
What makes TAPS buffer ideal for capillary electrophoresis?
TAPS maintains a stable alkaline pH (7.7–9.1), making it well-suited for DNA analysis in capillary electrophoresis by preventing shifts in charge and ensuring accurate separation.
How does TAPS buffer compare to other Good’s buffers like Tris or HEPES?
TAPS has a higher pKa (8.44), making it better suited for alkaline applications, whereas Tris (pKa 8.1) is more commonly used for general biological applications, and HEPES (pKa 7.5) is optimal for physiological pH conditions.
Can TAPS buffer be used in enzyme assays and cell culture media?
Yes! TAPS is widely used in enzyme assays due to its minimal interference with biomolecules and in cell culture media to maintain stable pH conditions in alkaline environments.
