MOPS buffer (3-(N-morpholino)propanesulfonic acid) is a widely used Good’s buffer, ideal for maintaining stable, near-neutral pH in biological and biochemical applications. With a pKa of 7.20 and an effective pH range of 6.5 to 7.9, MOPS provides precise buffering for RNA electrophoresis, protein purification, and chromatography. It is also a preferred running buffer for polyacrylamide gel electrophoresis (PAGE), ensuring reliable separation and analysis of nucleic acids and proteins.
Strong pH stability for accurate experimental conditions
Ideal for RNA electrophoresis and gel-based separation techniques
Minimal metal ion binding for compatibility with biological assays
MOPS buffer supports high-resolution molecular biology workflows, making it a trusted choice for researchers working with nucleic acids and proteins in electrophoresis, chromatography, and biochemical assays.
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Frequently Asked Questions
Why is MOPS buffer commonly used in RNA electrophoresis?
MOPS buffer maintains a stable pH in the 6.5–7.9 range, preventing RNA degradation and enabling accurate separation in agarose gel electrophoresis.
How does MOPS compare to other Good’s buffers like HEPES or MES?
MOPS is best suited for near-neutral pH applications, while HEPES is optimal for physiological conditions, and MES is preferred for slightly acidic environments. The right choice depends on your experimental pH requirements.
Can MOPS buffer be used in protein purification and chromatography?
Yes, MOPS is highly compatible with protein purification techniques, offering minimal interference in chromatography-based separation methods.
