Maintain precise pH control in biological research with HEPES buffer, a trusted Good’s buffer for physiological conditions. With a pKa of 7.5 at 25°C, HEPES effectively buffers in the pH range of 6.8 to 8.2, making it an excellent choice for cell culture, enzyme assays, and protein purification—especially when CO₂-based buffering systems are unsuitable.
Strong pH stability for reliable experimental conditions
Resistant to degradation under various environmental conditions
Minimal metal ion interaction ensures compatibility with sensitive biological systems
Due to its chemical stability and non-toxic properties, HEPES buffer is ideal for long-term cell culture studies, biochemical assays, and applications requiring precise pH maintenance without COâ‚‚ buffering.
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Frequently Asked Questions
Why is HEPES buffer preferred over bicarbonate buffers in cell culture?
HEPES provides stable pH buffering without relying on COâ‚‚ levels, making it ideal for experiments conducted outside of COâ‚‚-regulated incubators.
How do I prepare a HEPES buffer solution?
Dissolve the required amount of HEPES in deionized water, adjust the pH with NaOH or HCl, and filter-sterilize if necessary. The optimal concentration depends on your specific application.
Does HEPES buffer interact with biological molecules or enzymes?
HEPES exhibits minimal interaction with biomolecules, enzymes, and metal ions, ensuring compatibility with a wide range of biochemical and cellular assays.
