Tris-EDTA Buffer, TE (20X, pH 7.4, Nuclease Free)
CAT#: BM-302-DR
- Useful in biochemistry and molecular biology techniques
- Effective as a storage buffer to maintain integrity of nucleic acid samples
- Ready-to-use 20X solution, providing a buffering system within the pH range of 7.5-8.5
Available sizes:
Product Description
The Tris-EDTA (TE) Buffer is a commonly used buffer solution in molecular biology, especially in processes involving nucleic acids. This buffer is composed of Tris, a buffering agent, and EDTA, which has the ability to prevent degradation of DNA and RNA by chelating magnesium or other divalent metal ions, and allowing the pH regulation within the range of 7.5-8.5. The TE Buffer is widely used often used for DNA and RNA extraction, electrophoresis, and for DNA automated sequencing.
Specifications
Method: | Filtered through 0.22-micron filter. |
---|---|
Storage: | Room Temp. |
Synonym: | TE, Tris EDTA Solution |
pH: | 7.40 ± 0.15 (1X) |
QC Testing: | Appearance, pH, DNase Free, RNase Free |
Component Name | CAS | Molecular Weight | Concentration |
---|---|---|---|
Tris-Base | 77-86-1 | 121.14 | 200 mM |
EDTA Disodium Salt Dihydrate | 6381-92-6 | 372.24 | 20 mM |
Hydrochloric Acid | 7647-01-0 | 36.46 | For pH Adjustment |
Water | 7732-18-5 | 18.02 | Rest |