Tris-Acetate Buffer (0.2 M, pH 7.4)
CAT#: BB-2410
- Useful in cell culture, biochemistry, and molecular biology techniques
- Effective in pH regulation within nucleic acid purification and DNA mobility studies
- Ready-to-use solution, providing a buffering system within the pH range of 6.0-8.0
Available sizes:
Product Description
The Tris-Acetate Buffer is a buffering agent composed of Tris-base and acetic acid. The combination of these ingredients yields a pH range of 6.0-8.0. This buffer is ideal for requiring a pH slightly above physiological conditions. Often used to support the electrophoresis of nucleic acids on agarose gel and protein electrophoresis employing SDS-PAGE and related blotting techniques.
Specifications
| Method: | Filtered through 0.22-micron filter. |
|---|---|
| Storage: | Room Temp. |
| pH: | 7.40 ± 0.20 |
| QC Testing: | Appearance, pH |
| Component Name | CAS | Molecular Weight | Concentration |
|---|---|---|---|
| Tris-Base | 77-86-1 | 121.14 | 200 mM |
| Acetic Acid, Glacial | 64-19-7 | 60.05 | For pH adjustment |
| Water | 7732-18-5 | 18.02 | Rest |
Product Citations
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