Experience precise separation on your HPLC with quality buffers
Whether you use HPLC for analytics or as a means to purify compounds for downstream assays, you've likely experienced issues with your chromatograms. Is your baseline rising? Experiencing low sensitivity? Spikes or noise attributing to poorly resolved bands? This can be attributed to your buffer conditions or quality. See how we can help.
The Process
HPLC's excellence lies in its ability to deliver high-resolution, reproducible results through advanced column technology and precise solvent delivery. This technique, a blend of advanced column technology and exact solvent delivery, hinges on top-tier buffer quality to ensure unparalleled separation efficiency. It's the backbone of groundbreaking discoveries, demonstrating the synergy of meticulous methodology and superior chemical components.
How we simplify the process
Boston BioProducts can help with your tricky HPLC runs. We offer expert consultations to help you identify the factors contributing to those peaks that are blending together on a noisy chromatogram. We can help you minimize noise and improve sensitivity with our supply of high quality, contaminant-free, HPLC buffers and expert insights. Boston BioProducts is by your side to improve separation with our offering of precise and appropriate pH conditions and salt gradients for your ion-exchange chromatography. Our proven history of success and rigorous quality assurance processes ensure your needs are met at every step of the way.
Ways Boston BioProducts can help
Efficiently troubleshoot HPLC through expert consultations
Are you unable to identify what is causing your low-quality chromatograms? Can't pinpoint the right HPLC buffers conditions? Our team of HPLC experts are experienced in helping you identify optimal buffer conditions, such as the appropriate pH and salt gradient, to achieve the best analyte separation. We suggest using only the highest quality buffers to prevent contamination issues.
Boost signal and minimize noise with high-purity HPLC buffers
If you are experiencing noisy chromatograms or the appearance of spurious peaks, the likely culprit is contamination in your buffers. Boston BioProducts offers HPLC grade solvents, salts, and acid/base modifiers. As the true manufacturer of each of our buffers, we ensure the quality of our products through our proactive quality culture, and our reliable vendor and distributor network.
Optimize separation with custom HPLC buffers manufacturing
Not all HPLC experiments are made equal. Tricky analytes lead to the need for specific buffer conditions to achieve optimal separation. Do you need buffers at precise and specific pH for your ion-exchange chromatography? Are your salt gradients tricky to make? Specify the recipe and specific conditions for your HPLC needs, and Boston BioProducts will take care of the rest. Our state-of-the-art quality driven facilities ensure a quality, scalable custom product.
Learn more about how
Boston BioProducts can help
Featured Products for HPLC Protein Purification
Featured Services & Resources
Custom Buffer Manufacturing
We manufacture virtually any buffer, so you don't have to. Let us focus on providing high-quality, reproducible buffers so you can focus on your science.
Quality Assurance
Our products provide a strong foundation for your science. Boston BioProducts supplies HPLC buffers you can trust, with comprehensive quality documentation.
Our Facilities
We manufacture all of our HPLC buffers in-house at our ISO-certified commercial manufacturing facility. Our facilities are built with quality in mind, ensuring that only materials ready to be shipped, are shipped.
Related Applications
IHC Buffers
Plasmid Isolation
Frequently Asked Questions
Choosing an appropriate buffer for HPLC depends entirely on the method being used for separation. Usually during reverse phase separation, it's important to use a buffer with a pKa close to the pH of the buffer, preferably less than two units of the pH of the mobile phase.
In reverse phase HPLC separation, the pH of the buffer used changes the ionization of molecules in the sample. With nonpolar analytes, the change is small, and is much greater for polar molecules. Changes in analyte ionization will change the degree of separation and retention times of the molecules.
Buffers are used in HPLC to mitigate the influence of ionization of acidic or basic functional groups of analytes being separated. The buffer reduces fluctuations of pH, which would consequently cause unwanted influence on separation of the analytes.
The buffers used during HPLC influence the separation of analytes by influencing the binding to the stationary phase. Changing the buffer conditions will ultimately change the retention time of compounds to the stationary phase, and influence effective separation. This is why choosing the right buffer is so important.
Buffers are capable of maintaining pH by neutralizing acidic and basic ions. This maintains a specific pH, causing minimal shift despite the addition of compounds. This is important during chromatography because changes in pH could cause drastic differences in analyte separation.