qPCR DNase Buffer (10X)
CAT#: QP-120
- Useful in qPCR protocols
- Supports activity of DNase Enzymes, ensuring proper DNA digestion
- Ready-to-use solution tested for pH
Available sizes:
Product Description
The qPCR DNase Buffer enhances the activity of DNase enzymes, ensuring efficient DNA digestion. It facilitates the denaturation of cellular proteins and membranes, allowing DNase to access and degrade DNA substrates. This process is necessary for accurate qPCR analysis, as it helps release necessary nucleotides and minimizes inhibitors that could affect the qPCR reaction. This ensures high sensitivity and specificity in detecting target sequences.
Specifications
| Method: | Filtered through 0.22-micron filter. |
|---|---|
| Storage: | Room Temp. |
| Synonym: | DNase Reagent, qPCR Buffer |
| pH: | 7.50 ± 0.15 (1X) |
| QC Testing: | Appearance, pH |
| Component Name | CAS | Molecular Weight | Concentration |
|---|---|---|---|
| Tris-Base / Tris-HCl | 77-86-1 / 1185-53-1 | 121.12 / 157.6 | 10.31 mM |
| Magnesium Chloride Hexahydrate | 7791-18-6 | 203.3 | 10.31 mM |
| Calcium Chloride Dihydrate | 10035-04-8 | 147.01 | 10.31 mM |
| Water | 7732-18-5 | 18.02 | Rest |
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