Tris-Buffered Saline-Tween, TBST
CAT#: BPS-321
- For use in bioprocessing and biomolecule purification procedures
- Buffer-surfactant for use as a diluent, within washing procedures, and within molecular biology
- Formulated with USP Compendial-Grade Raw Materials and tested for pH, Conductivity, Osmolality, Endotoxin, DNase, RNase, Protease
Available sizes:
Product Description
Tris Buffered Saline Tween (TBST) is a buffer-surfactant solution supporting a stable pH range between 7.4 and 8.0. When combined in a variable ratio, TBST is an effective wash buffer in Western blotting, in situ hybridization procedures, and immunohistochemistry staining, as it minimizes non specific binding of the materials without disrupting antigen-antibody binding. TBST is also used as an antibody diluent in immunoassays, supports an array of molecular biology techniques, and supports bioprocessing protocols. This TBST is formulated in WFI-Quality Water.
Specifications
| Method: | Filtered through 0.22-micron filter. |
|---|---|
| Storage: | Room Temp. |
| Synonym: | Western blotting wash buffer |
| pH: | 7.40 ± 0.20 |
| QC Testing: | Appearance, pH, Conductivity, Osmolality, Low Endotoxin, DNase Free, RNase Free, Protease Free |
| Component Name | CAS | Molecular Weight | Concentration |
|---|---|---|---|
| Tris-Base / Tris-HCl | 77-86-1 / 1185-53-1 | 121.14 / 157.60 | 50 mM |
| Sodium Chloride | 7647-14-5 | 58.44 | 150 mM |
| Tween-20 | 9005-64-5 | ~1228 | 0.05% (v/v) |
| Water for Injection (WFI) | 7732-18-5 | 18.02 | Rest |
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