Tris Buffered Saline-Tween, TBST (10X, with 0.5% Tween-20, pH 7.6)
CAT#: IBB-181-6
- Useful in cell culture, molecular biology, protein research, and purification
- Often used as a wash buffer in immunoassays and protein purification
- Ready-to-use 10X solution, providing a buffering system within the pH range of 7.4-8.0
Available sizes:
Product Description
10X Tris Buffered Saline - Tween (TBST) is a buffer-surfactant solution supporting a stable pH range between 7.4 and 8.0. When combined in a variable ratio, TBST is an effective wash buffer in Western blotting, in situ hybridization procedures, and immunohistochemistry staining, as it minimizes non specific binding of the materials without disrupting antigen-antibody binding. TBST is also used as an antibody diluent in immunoassays, and supports an array of molecular biology techniques.
Specifications
| Method: | Filtered through 0.22-micron filter. |
|---|---|
| Storage: | Room Temp. |
| Synonym: | TBST |
| pH: | 7.60 ± 0.20 (1X) |
| QC Testing: | Appearance, pH |
| Notes: | To make 1X, dilute 10-fold with deionized water. |
| Component Name | CAS | Molecular Weight | Concentration |
|---|---|---|---|
| Tris-Base / Tris-HCl | 77-86-1 / 1185-53-1 | 121.14 / 157.60 | 500 mM |
| Sodium Chloride | 7647-14-5 | 58.44 | 1.5 M |
| Tween-20 | 9005-64-5 | ~1228 | 0.50% |
| Water | 7732-18-5 | 18.02 | Rest |
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