Tris Buffered Saline, TBS (20X, for Western Blot Washing)
CAT#: BM-301X
- Useful in cell culture, molecular biology, protein research, and purification
- Often used as a wash buffe in blotting procedures
- Ready-to-use 20X solution, providing a buffering system within the pH range of 7.4-8.0
Available sizes:
Product Description
The Tris Buffered Saline (TBS) is an isotonic non-toxic buffer solution used commonly in biochemical applications. Tris maintains a stable pH range typically between 7.4 to 8.0 while NaCl regulates osmotic balance via its elected concentration. This TBS Solution is formulated at a 20X concentration, and is useful for washing in Western Blotting.
Specifications
Method: | Filtered through 1-micron filter. |
---|---|
Storage: | Room Temp. |
Synonym: | TBS |
pH: | 7.40 ± 0.20 (1X) |
QC Testing: | Appearance, pH |
Notes: | To make 1X, dilute 20-fold with deionized water. |
Component Name | CAS | Molecular Weight | Concentration |
---|---|---|---|
Tris-Base / Tris-HCl | 77-86-1 / 1185-53-1 | 121.14 / 157.60 | 1 M |
Sodium Chloride | 7647-14-5 | 58.44 | 3 M |
Water | 7732-18-5 | 18.02 | Rest |