Tricine Buffer (0.2 M, pH 6.5)
CAT#: BB-199
- Useful in protein research, purification, biochemistry, and molecular biology
- Supports cell resuspension in cell culture and liquid chromatography techniques
- Ready-to-use solution tested for pH
Available sizes:
Product Description
Tricine [N-tris(Hydroxymethyl)methylglycine] is a zwitterionic Good's buffers. It belongs to the Tris family of buffers and is used in biochemistry and molecular biology applications. The Tricine buffer is commonly used in SDS-PAGE to separate polypeptides that are in the molecular weight range of 1 to 100 kDa. It is also used in capillary zone electrophoresis, high performance liquid chromatography and ion exchange chromatography, and the resuspension of cells.
Specifications
| Method: | Filtered through 0.22-micron filter. |
|---|---|
| Storage: | 2-8°C |
| pH: | 6.50 ± 0.15 |
| QC Testing: | Appearance, pH |
| Component Name | CAS | Molecular Weight | Concentration |
|---|---|---|---|
| Tricine | 5704-04-1 | 179.17 | 200 mM |
| Sodium Hydroxide | 1310-73-2 | 40 | For pH Adjustment |
| Water | 7732-18-5 | 18.02 | Rest |
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