Stripping Buffer (4X, Medium)
CAT#: BP-98
- Useful in biochemistry and molecular biology techniques
- Can be used to strip antibodies from Western blot membranes with minimal damage to immobilized proteins
- Ready-to-use solution tested for pH
Available sizes:
Product Description
The 4X Stripping Buffer (Medium) is a less stringent stripping solution comprising of glycine and the detergents such as SDS and Tween-20. It is used for breaking antibody-antigen interactions, resulting in removal of primary and secondary antibodies from probed Western blot membranes without significantly affecting the immobilized proteins. This solution enables reprobing Westen blot membranes several times with different antibodies, preventing the need for running multiple gels and transfers.
Specifications
| Method: | Filtered through 1-micron filter. |
|---|---|
| Storage: | Room Temp. |
| pH: | 2.50 ± 0.20 (1X) |
| QC Testing: | Appearance, pH |
| Component Name | CAS | Molecular Weight | Concentration |
|---|---|---|---|
| Glycine | 56-40-6 | 75.07 | 400 mM |
| Sodium Dodecyl Sulfate (SDS) [20% Solution] | 151-21-3 | 288.38 | 0.20% |
| Tween-20 | 9005-64-5 | ~1228 | 2% |
| Hydrochloric Acid (6 N) | 7647-01-0 | 36.46 | For pH Adjustment |
| Water | 7732-18-5 | 18.02 | Rest |
Product Citations
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