Stacking Buffer
CAT#: BP-95
- Useful in biochemistry and molecular biology techniques
- Can be used to prepare the stacking gel in SDS-PAGE protocols
- Ready-to-use solution tested for pH
Available sizes:
Product Description
When casting polyacrylamide protein gels, the Stacking Buffer is used to create the stacking portion of SDS or native gels. Its key features include facilitating the concentration and stacking of sample molecules at the interface between the stacking gel and the resolving gel, ensuring sharp, well-defined bands during electrophoresis. Additionally, the buffer maintains a stable pH, typically around 6.8, protecting analytes from pH fluctuations.
Specifications
| Method: | Filtered through 1-micron filter. |
|---|---|
| Storage: | Room Temp. |
| pH: | 6.80 ± 0.20 |
| QC Testing: | Appearance, pH |
| Component Name | CAS | Molecular Weight | Concentration |
|---|---|---|---|
| Tris-Base / Tris-HCl | 77-86-1 / 1185-53-1 | 121.14 / 157.60 | 500 mM |
| Sodium Dodecyl Sulfate (SDS) [20% Solution] | 151-21-3 | 288.38 | 0.40% |
| Water | 7732-18-5 | 18.02 | Rest |
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Separating (Resolving) Buffer- BP-90Sku#: BP-90