Semi Dry Blot Transfer Buffer (10X)
CAT#: BP-194
- Useful in biochemistry and molecular biology techniques
- Often used with semi-dry transfer units to facilitate the efficient transfer of proteins from gels to membranes
- Ready-to-use solution tested for pH
Available sizes:
Product Description
The Semi-Dry Blot Transfer Buffer is used in the semi-dry transfer method of Western blotting. This buffer has a lower ionic strength than traditional Wet Transfer Buffers, which allows for a faster transfer with electric current, minimizing the heat generated during the transfer process. This buffer is adjusted to be slightly alkaline, around pH 8.3, promoting efficient protein transfer while preventing denaturation.
Specifications
| Method: | Filtered through 1-micron filter. |
|---|---|
| Storage: | Room Temp. |
| pH: | > 8.00 |
| QC Testing: | Appearance, pH |
| Notes: | To prepare 1 L of transfer buffer for use, dilute 100 mL of this solution and 200 mL of Methanol in 700 mL of deionized water. |
| Component Name | CAS | Molecular Weight | Concentration |
|---|---|---|---|
| Tris-Base | 77-86-1 | 121.14 | 480 mM |
| Glycine | 56-40-6 | 75.07 | 390 mM |
| Sodium Dodecyl Sulfate (SDS) [20% Solution] | 151-21-3 | 288.38 | 0.375% (w/v) |
| Water | 7732-18-5 | 18.02 | Rest |
Product Citations
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