Saponin Cell Permeating Solution (0.5% w/v in PBS)
CAT#: CLA-120
Saponin Cell Permeating Solution is a reagent used to permeabilize membranes, while keeping the membrane structure intact. Membrane permeabilization is a necessary step for antigen detection, dyeing procedures, and various other cellular analyses. During pore formation, Saponin targets cholesterols within the cellular membrane, acting as a more specific permeabilization agent than other comparable detergents.Â
Available sizes:
Product Description
- Useful in flow cytometry and various intracellular analyses.
- Permeabilizes cell membranes by targeting cholesterol molecules within the membrane
- Provided at 10X concentrate and is ready-to-use once diluted to a 1X working concentration.
Specifications
| Method: | Filtered through 0.22-micron filter. |
|---|---|
| Storage: | Room Temp. |
| pH: | 7.40 ± 0.20 |
| QC Testing: | Appearance, pH |
| Component Name | CAS | Molecular Weight | Concentration |
|---|---|---|---|
| Saponin | 8047-15-2 | N/A | 0.5% (v/v) |
| Sodium Phosphate Dibasic Anhydrous | 7558-79-4 | 141.96 | 10.1 mM |
| Potassium Phosphate Monobasic Anhydrous | 7778-77-0 | 136.09 | 1.76 mM |
| Potassium Chloride | 7447-40-7 | 74.55 | 2.7 mM |
| Sodium Chloride | 7647-14-5 | 58.44 | 137 mM |
| Water | 7732-18-5 | 18.02 | Rest |
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