RIPA Buffer (pH 8.0)
CAT#: BP-116
|
- Useful in cell lysis and extraction protocols
- Effective in the full solubilization of cellular compartments and disruption of proteins
- Ready-to-use solution tested for pH
Available sizes:
Product Description
The RIPA Buffer is used for rapid, efficient cell lysis and solubilization of proteins from adherent and suspension cultured mammalian cells and tissue samples. With the combined action of its ionic detergents, the RIPA buffer is extremely useful for disruption of all proteins within cellular compartments, including nuclear, membrane, and mitochondria. This buffer is compatible with many downstream applications, such as SDS-PAGE, immunoprecipitation, Western blot, and ELISA assays.
Specifications
| Method: | Filtered through 0.22-micron filter. |
|---|---|
| Storage: | 2-8°C |
| Synonym: | RIPA |
| pH: | 8.00 ± 0.20 |
| QC Testing: | Appearance, pH |
| Notes: | For long term storage, store product at -20°C. |
| Component Name | CAS | Molecular Weight | Concentration |
|---|---|---|---|
| Tris-Base / Tris-HCl | 77-86-1 / 1185-53-1 | 121.14 / 157.60 | 50 mM |
| Sodium Chloride | 7647-14-5 | 58.44 | 150 mM |
| Nonidet P-40 Substitute (NP-40) | 9016-45-9 | ~680 | 1% |
| Sodium Deoxycholate (10% Solution) | 302-95-4 | 414.55 | 0.50% |
| Sodium Dodecyl Sulfate (SDS) [20% Solution] | 151-21-3 | 288.38 | 0.10% |
| Water | 7732-18-5 | 18.02 | Rest |
Product Citations
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