RIPA Buffer (5X)
CAT#: BP-115-5X
- Useful in cell lysis and extraction protocols
- Effective in the full solubilization of cellular compartments and disruption of proteins
- Ready-to-use 5X solution tested for pH
Available sizes:
Product Description
The 5X RIPA Buffer is used for rapid, efficient cell lysis and solubilization of proteins from adherent and suspension cultured mammalian cells and tissue samples. With the combined action of its ionic detergents, the RIPA buffer is extremely useful for disruption of all proteins within cellular compartments, including nuclear, membrane, and mitochondria. This buffer is compatible with many downstream applications, such as SDS-PAGE, immunoprecipitation, Western blot, and ELISA.
Specifications
| Method: | Filtered through 0.22-micron filter. |
|---|---|
| Storage: | 2-8°C |
| Synonym: | RIPA |
| pH: | 7.40 ± 0.20 (1X) |
| QC Testing: | Appearance, pH |
| Notes: | For long term storage, store product at -20°C. |
| Component Name | CAS | Molecular Weight | Concentration |
|---|---|---|---|
| Tris-Base / Tris-HCl | 77-86-1 / 1185-53-1 | 121.14 / 157.60 | 250 mM |
| Sodium Chloride | 7647-14-5 | 58.44 | 750 mM |
| Nonidet P-40 (IGEPAL CA-630) | 9002-93-1 | N/A | 5% (v/v) |
| Sodium Deoxycholate (10% Solution) | 302-95-4 | 414.55 | 2.5% (w/v) |
| Sodium Dodecyl Sulfate (SDS) [20% Solution] | 151-21-3 | 288.38 | 0.5% (w/v) |
| Water | 7732-18-5 | 18.02 | Rest |
Product Citations
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RIPA Buffer (2X, with Triton X & Glycerol)- BP-411Sku#: BP-411
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RIPA Buffer- BP-115Sku#: BP-115
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RIPA Buffer (with Triton X)- BP-116TXSku#: BP-116TX
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RIPA Buffer (2X, pH 8.0)- BP-116XSku#: BP-116X
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RIPA Buffer (2X)- BP-115XSku#: BP-115X