RIPA Buffer (2X, with Triton X & Glycerol)
CAT#: BP-411
- Useful in cell lysis and extraction protocols
- Effective in the full solubilization of cellular compartments and disruption of proteins
- Ready-to-use 2X solution tested for pH
Available sizes:
Product Description
The 2X RIPA Buffer is used for rapid, efficient cell lysis and solubilization of proteins from adherent and suspension cultured mammalian cells and tissue samples. This buffer is extremely useful for disruption of all proteins within cellular compartments, including nuclear, membrane, and mitochondrial proteins and is compatible with many downstream applications, such as SDS-PAGE, immunoprecipitation, Western blot, and ELISA assays. This product is made with Triton X and Glycerol.
Specifications
| Method: | Filtered through 0.22-micron filter. |
|---|---|
| Storage: | 2-8°C |
| Synonym: | Extraction Buffer, RIPA Buffer, RIPA Lysis Buffer |
| pH: | 7.40 ± 0.15 (1X) |
| QC Testing: | Appearance, pH |
| Component Name | CAS | Molecular Weight | Concentration |
|---|---|---|---|
| Tris-Base / Tris-HCl | 77-86-1 / 1185-53-1 | 121.14 / 157.6 | 100 mM |
| Sodium Chloride | 7647-14-5 | 58.44 | 300 mM |
| Triton X-100 | 9036-19-5 | ~625 | 2% (v/v) |
| Glycerol | 56-81-5 | 92.09 | 10% (v/v) |
| Sodium Deoxycholate (10% Solution) | 302-95-4 | 414.55 | 1% (w/v) |
| Sodium Dodecyl Sulfate (SDS) [20% Solution] | 151-21-3 | 288.38 | 0.2% (w/v) |
| Water | 7732-18-5 | 18.02 | Rest |
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