PIPES Buffer (0.5 M, pH 7.5)
CAT#: BB-2270
- Useful in protein research, purification, biochemistry, and molecular biology
- Supports electron microscopy, protein crystallization, and chromatography techniques while regulating pH
- Ready-to-use solution, providing a buffering system within the pH range of 6.1-7.5
Available sizes:
Product Description
The PIPES Buffer is a zwitterionic buffering agent commonly used in biochemistry and molecular biology for its ability to maintain a stable pH. With a pKa of approximately 6.8, PIPES is effective in the pH range of 6.1 to 7.5, making it suitable for cell culture, enzyme assays, protein purification, and as a running buffer in gel electrophoresis. PIPES is also used in electron microscopy, protein crystallization, and isoelectric focusing and chromatography.
Specifications
| Method: | Filtered through 0.22-micron filter. |
|---|---|
| Storage: | 2-8°C |
| Synonym: | PIPES |
| pH: | 7.50 ± 0.20 |
| QC Testing: | Appearance, pH |
| Notes: | Slight coloration with time will not affect the quality of the product |
| Component Name | CAS | Molecular Weight | Concentration |
|---|---|---|---|
| PIPES | 5625-37-6 | 302.37 | 500 mM |
| Sodium Hydroxide | 1310-73-2 | 40 | For pH Adjustment |
| Water | 7732-18-5 | 18.02 | Rest |
Product Citations
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PIPES Buffer (0.5 M, pH 6.8, pH Adjusted with KOH)- BB-121-KSku#: BB-121-K
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PIPES Buffer (0.5 M, pH 6.8)- BB-121Sku#: BB-121
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PIPES Buffer (0.5 M, pH 7.0)- BB-122Sku#: BB-122
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PIPES Buffer (1 M, pH 6.8)- BB-2280Sku#: BB-2280
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PIPES Buffer (1 M, pH 7.0)- BB-2284Sku#: BB-2284