PIPES Buffer (0.5 M, pH 7.5)

CAT#: BB-2270
91 /100
10 Citations
Bioz Stars Product Rating
The world's only objective ratings for scientific research products
Mentions 
Recency 
View product citations >
  • Useful in protein research, purification, biochemistry, and molecular biology
  • Supports electron microscopy, protein crystallization, and chromatography techniques while regulating pH
  • Ready-to-use solution, providing a buffering system within the pH range of 6.1-7.5

Available sizes:

CAT#:
BB-2270-100ml Before requesting a quote or making a purchase, please make sure you select the correct size.
Unit Price:
$42.00 / 100ml
Total (USD)
$42.00
Do you need a custom reagent?

Build your own buffer

Product Description

The PIPES Buffer is a zwitterionic buffering agent commonly used in biochemistry and molecular biology for its ability to maintain a stable pH. With a pKa of approximately 6.8, PIPES is effective in the pH range of 6.1 to 7.5, making it suitable for cell culture, enzyme assays, protein purification, and as a running buffer in gel electrophoresis. PIPES is also used in electron microscopy, protein crystallization, and isoelectric focusing and chromatography.

Specifications

More Information
Method: Filtered through 0.22-micron filter.
Storage: 2-8°C
Synonym: PIPES
pH: 7.50 ± 0.20
QC Testing: Appearance, pH
Notes: Slight coloration with time will not affect the quality of the product
Component NameCASMolecular WeightConcentration
PIPES 5625-37-6 302.37 500 mM
Sodium Hydroxide 1310-73-2 40 For pH Adjustment
Water 7732-18-5 18.02 Rest