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PIPES Buffer (0.5 M, pH 6.8, pH Adjusted with KOH)
CAT#: BB-121-K
- Useful in protein research, purification, biochemistry, and molecular biology
- Supports electron microscopy, protein crystallization, and chromatography techniques while regulating pH
- Ready-to-use solution, providing a buffering system within the pH range of 6.1-7.5
Available sizes:
Product Description
The PIPES Buffer is a zwitterionic buffering agent commonly used in biochemistry and molecular biology for its ability to maintain a stable pH. With a pKa of approximately 6.8, PIPES is effective in the pH range of 6.1 to 7.5, making it suitable for cell culture, enzyme assays, protein purification, and as a running buffer in gel electrophoresis. PIPES is also used in electron microscopy, protein crystallization, and isoelectric focusing and chromatography.
Specifications
| Method: | Filtered through 0.22-micron filter. |
|---|---|
| Storage: | 2-8°C |
| Synonym: | PIPES |
| pH: | 6.80 ± 0.20 |
| QC Testing: | Appearance, pH |
| Component Name | CAS | Molecular Weight | Concentration |
|---|---|---|---|
| PIPES | 5625-37-6 | 302.37 | 500 mM |
| Potassium Hydroxide (Pellets) | 1310-58-3 | 56.11 | For pH Adjustment |
| Water | 18.02 | 18.02 | Rest |
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