Phosphate Buffered Saline-Tween, PBST (20X, with 2% Tween-20, pH 7.4)
CAT#: IBB-170X
- Useful in cell culture, molecular biology, protein research, and purification
- Often used as a wash buffer in immunoassays and protein purification
- Ready-to-use 20X solution, providing a buffering system within the pH range of 7.0-7.4
Available sizes:
Product Description
20X Phosphate Buffered Saline with Tween-20 (PBST) is a specialized variant of PBS, supplemented with Tween-20, a non-ionic surfactant. Tween-20 minimizes the non-specific binding and background noise, resulting in enhanced signal to noise ratio. PBST is often used in immunoassays such as ELISA and Western Blotting, where it serves as a washing and blocking buffer. With a stable buffering pH range between 7.0 and 7.4, PBST maintains ionic strength and protein solubility for biological assays.
Specifications
| Method: | Filtered through 0.22-micron filter. |
|---|---|
| Storage: | Room Temp. |
| Synonym: | PBST |
| pH: | 7.40 ± 0.20 (1X) |
| QC Testing: | Appearance, pH |
| Notes: | To make 1X, dilute 20-fold with deionized water. In case of crystal formation, warm the contents until all the crystals are dissolved. |
| Component Name | CAS | Molecular Weight | Concentration |
|---|---|---|---|
| Sodium Phosphate Dibasic Anhydrous | 7558-79-4 | 141.96 | 202 mM |
| Potassium Phosphate Monobasic Anhydrous | 7778-77-0 | 136.09 | 35.2 mM |
| Potassium Chloride | 7447-40-7 | 74.55 | 54 mM |
| Sodium Chloride | 7647-14-5 | 58.44 | 2.74 M |
| Tween-20 | 9005-64-5 | ~1228 | 2% (v/v) |
| Water | 7732-18-5 | 18.02 | Rest |
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