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Phosphate Buffered Saline, PBS (10X, for Western Blot Washing)
CAT#: BM-222
- Useful in cell culture, molecular biology, protein research, and purification
- Often used as a wash buffer during western blot protocols
- Ready-to-use solution, providing a buffering system within the pH range of 7.0-7.4
Available sizes:
Product Description
Phosphate Buffered Saline (PBS) is a versatile buffer solution widely used in biological research due to its isotonic and non-toxic nature to cells. With a pH range of 7.0 to 7.4, PBS mimics the osmolarity and ion concentrations of the human body, making it ideal for a variety of applications. This product functions as wash buffer in Western blotting and immunoassays, and is formulated in a 10X concentration. Dilute to desired concentration.
Specifications
| Method: | Filtered through 1-micron filter. |
|---|---|
| Storage: | Room Temp. |
| Synonym: | PBS |
| pH: | 7.40 ± 0.20 (1X) |
| QC Testing: | Appearance, pH |
| Notes: | To make 1X, dilute 10-fold with deionized water. |
| Component Name | CAS | Molecular Weight | Concentration |
|---|---|---|---|
| Sodium Phosphate Monobasic Anhydrous (Monosodium Phosphate) / Sodium Phosphate Dibasic Anhydrous | 7558-80-7 / 7558-79-4 | 119.98 / 141.96 | 100 mM |
| Sodium Chloride | 7647-14-5 | 58.44 | 1.5 M |
| Water | 7732-18-5 | 18.02 | Rest |
Product Citations
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Phosphate Buffered Saline, PBS (10X, RNase Free)- BM-220-DRSku#: BM-220-DR
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Phosphate-Buffered Saline, PBS (10X, Low Endotoxin)- BM-220-LTSku#: BM-220-LT
