NP-40 Lysis Buffer
CAT#: BP-119
- Useful in cell lysis and extraction protocols
- Successfully lysis cell membranes and partially lyses nuclear membranes without full disruption
- Ready-to-use solution tested for pH
Available sizes:
Product Description
The NP-40 Lysis Buffer is a solubilizing agent used to prepare cell extracts for immunoassays and blotting techniques. As one of the most popular lysis buffers, the NP-40 lysis buffers can support a variety of laboratory workflows. Relying on the non-ionic detergent NP-40 as its primary solubilizing agent, this Lysis buffer is weaker than a RIPA buffer, and can permeabilize cell membranes, partially open the nucleus without fully disrupting it, and lyse mitochondrial membranes.
Specifications
| Method: | Filtered through 0.22-micron filter. |
|---|---|
| Storage: | 2-8°C |
| Synonym: | NP-40 |
| pH: | 7.40 ± 0.20 |
| QC Testing: | Appearance, pH |
| Notes: | For long term storage, store product at -20°C. |
| Component Name | CAS | Molecular Weight | Concentration |
|---|---|---|---|
| Tris-Base / Tris-HCl | 77-86-1 / 1185-53-1 | 121.14 / 157.60 | 50 mM |
| Sodium Chloride | 7647-14-5 | 58.44 | 150 mM |
| EDTA (0.5 M, pH 7.4) | 60-00-4 | 292.24 | 5 mM |
| Nonidet P-40 (IGEPAL CA-630) | 9002-93-1 | N/A | 1% |
| Water | 7732-18-5 | 18.02 | Rest |
Product Citations
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NP-40 Lysis Buffer (2X)- BP-119XSku#: BP-119X