MES-SDS Buffer (20X)
CAT#: BP-177
- Useful in biochemistry and molecular biology techniques
- Often used as a running buffer in SDS-PAGE to separate proteins based on their molecular weight
- Ready-to-use solution tested for pH
Available sizes:
Product Description
The 20X MES-SDS Running Buffer is formulated specifically for running proteins on Bis-Tris gels for separating small to medium-sized proteins under denaturing conditions in SDS-PAGE. When reconstituted from the 20X concentrate, this buffer maintains a stable pH of approximately 7.3. The MES-SDS Running Buffer enables faster and efficient resolution of well-defined protein bands in comparison to other running buffers in SDS-PAGE experiments.
Specifications
| Method: | Filtered through 1-micron filter. |
|---|---|
| Storage: | Room Temp. |
| Synonym: | MES |
| pH: | 7.30 ± 0.20 (1X) |
| QC Testing: | Appearance, pH |
| Notes: | To make 1X, dilute 20-fold with deionized water. Slight coloration with time will not affect the quality of this product. |
| Component Name | CAS | Molecular Weight | Concentration |
|---|---|---|---|
| MES Anhydrous | 4432-31-9 | 195.24 | 1M |
| Tris-Base | 77-86-1 | 121.14 | 1M |
| Sodium Dodecyl Sulfate (SDS) [20% Solution] | 151-21-3 | 288.38 | 2% |
| EDTA Disodium Salt Dihydrate | 6381-92-6 | 372.24 | 20 mM |
| Water | 7732-18-5 | 18.02 | Rest |
Product Citations
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