Imidazole Buffer (0.5 M, pH 7.0)

CAT#: BB-2114
  • Useful in protein research, biochemistry, and molecular biology applications
  • Effective in purification of histidine-tagged proteins, and within immunohistochemistry applications
  • Ready-to-use solution, providing a buffering system within the pH range of 6.2-7.8

Available sizes:

CAT#:
BP-177-500ml Before requesting a quote or making a purchase, please make sure you select the correct size.
Unit Price:
Total (USD)
This size requires a quote. Our team is here to assist you!
Do you need a custom reagent?

Build your own buffer

Product Description

The Imidazole Buffer is an aromatic heterocyclic diazole with a buffering pH range of 6.2 to 7.8. It holds applications in molecular biology, biochemistry, and proteomics. It can be employed as a component in buffers for horseradish peroxidase assays (HRP assays), as well as a chelator for binding various divalent cations. Additionally, Imidazole aids in the elution of histidine-containing proteins from divalent cation resins, employing immobilized metal affinity chromatography (IMAC).

Specifications

More Information
Method: Filtered through 0.22-micron filter.
Storage: Room Temp.
pH: 7.00 ± 0.20
QC Testing: Appearance, pH
Component NameCASMolecular WeightConcentration
Imidazole 288-32-4 68.08 500 mM
Hydrochloric Acid (6 N) 7647-01-0 36.46 For pH Adjustment
Water 7732-18-5 18.02 Rest

Related Products