HEPES Buffered Saline (5X, pH 7.4)
CAT#: BB-553
- Useful in cell culture, protein research, and resuspension
- Effective in regulating pH and osmotic pressure
- Ready-to-use 5X solution, providing a buffering system within the pH range of 6.8-8.2
Available sizes:
Product Description
HEPES [2-(4-(2-hydroxyethyl) piperazin-1-yl)ethanesulfonic acid] Buffered Saline (HBS) is a versatile buffer that maintains a stable pH in the range of 6.8 to 8.2, making it suitable for biological applications including in tissue culture, cell culture, cell isolation procedures, flow cytometry and for the preparation of plasmid DNA. It can also be used as a reagent for preparing transfection mixtures, and in protein isolation and activity studies.
Specifications
| Method: | Filtered through 0.22-micron filter. |
|---|---|
| Storage: | 2-8°C |
| Synonym: | Buffered Saline, HBSS, HEPES, HEPES BSS |
| pH: | 7.40 ± 0.15 (1X) |
| QC Testing: | Appearance, pH |
| Component Name | CAS | Molecular Weight | Concentration |
|---|---|---|---|
| HEPES | 7365-45-9 | 238.3 | 125 mM |
| Sodium Chloride | 7647-14-5 | 58.44 | 750 mM |
| Sodium Hydroxide | 1310-73-2 | 40 | For pH adjustment |
| Water | 7732-18-5 | 18.02 | Rest |
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HEPES Buffer (1 M, pH 7.6)- BBH-76Sku#: BBH-76
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HEPES Buffer (1 M, pH 7.5)- BBH-75Sku#: BBH-75
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HEPES Buffer (1 M, pH 7.0)- BBH-70Sku#: BBH-70
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Cell Culture Water- WT-045Sku#: WT-045
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Molecular Biology Grade Water- WT-020Sku#: WT-020
