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HEPES Buffered Saline (2X, for Transfection, Low Endotoxin)
CAT#: BM-165-LT
|
- Useful in cell culture, protein research, and resuspension
- Effective in regulating pH and osmotic pressure
- Ready-to-use 2X solution, providing a buffering system within the pH range of 6.8-8.2
Available sizes:
Product Description
HEPES [2-(4-(2-hydroxyethyl) piperazin-1-yl)ethanesulfonic acid] Buffered Saline is a versatile buffer that maintains a stable pH in the range of 6.8 to 8.2, making it suitable for biological applications including in tissue culture, cell culture, cell isolation procedures, and for the preparation of plasmid DNA. It can also be used as a reagent for preparing transfection mixtures, and in protein isolation and activity studies. This buffer is made at 2X concentration and tested for endotoxin.
Specifications
| Method: | Filtered through 0.22-micron filter. |
|---|---|
| Storage: | 2-8°C |
| Synonym: | HEPES |
| pH: | 7.00 ± 0.20 (1X) |
| QC Testing: | Appearance, pH, Low Endotoxin |
| Notes: | For long term storage, store product at -20°C. |
| Component Name | CAS | Molecular Weight | Concentration |
|---|---|---|---|
| HEPES | 7365-45-9 | 238.3 | 50 mM |
| Dextrose | 50-99-7 | 180.16 | 12 mM |
| Sodium Phosphate Dibasic Anhydrous | 7558-79-4 | 141.96 | 1.5 mM |
| Potassium Chloride | 7447-40-7 | 74.55 | 10 mM |
| Sodium Chloride | 7647-14-5 | 58.44 | 280 mM |
| Sodium Hydroxide | 1310-73-2 | 40 | For pH Adjustment |
| Water | 7732-18-5 | 18.02 | Rest |
Product Citations
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