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HEPES Buffer (1 M, pH 8.5)
CAT#: BBH-85
- Useful in protein research, purification, biochemistry, and molecular biology
- Supports cell culture, purification and enzyme studies, isoelectric focusing, bioprocessing protocols, and live cell imaging
- Ready-to-use solution, providing a buffering system within the pH range of 6.8-8.2
Available sizes:
Product Description
The HEPES [4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid)] Buffer is a versatile Good's zwitterionic buffering agent widely used in various applications in biochemistry and molecular biology. It maintains a stable pH in the range of 6.8 to 8.2, making it ideal for various applications at physiological pH, including cell culture, protein purification, isoelectric focusing, enzyme studies, bioprocessing, and live cell imaging.
Specifications
| Method: | Filtered through 0.22-micron filter. |
|---|---|
| Storage: | Room Temp. |
| Synonym: | C₈H₁₈N₂O₄S (aq), HEPES, HEPES Solution, 0.5M HEPES |
| pH: | 8.50 ± 0.20 |
| QC Testing: | Appearance, pH |
| Component Name | CAS | Molecular Weight | Concentration |
|---|---|---|---|
| HEPES | 7365-45-9 | 238.3 | 1 M |
| Sodium Hydroxide (Pellets) | 1310-73-2 | 40 | For pH adjustment |
| Water | 7732-18-5 | 18.02 | Rest |
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