HEPES Buffer (1 M, pH 7.5)
CAT#: BPS-336
- For use in bioprocessing and biomolecule purification procedures
- Buffering agent capable of regulating bioprocess environments at a physiological pH
- Formulated with USP Compendial-Grade Raw Materials and tested for pH, Conductivity, Osmolality, Endotoxin, DNase, RNase, Protease
Available sizes:
Product Description
The 1M HEPES [4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid)] Buffer is a zwitterionic buffering agent widely used in in biochemistry and molecular biology. It maintains a stable pH in the range of 6.8 to 8.2, making it ideal for various applications at physiological pH, including cell culture, protein purification, isoelectric focusing, enzyme studies, bioprocessing, and live cell imaging. This HEPES formulation is prepared in WFI-Quality water at a pH of 7.5, and is ideal for applications within Bioprocessing.
Specifications
| Method: | Filtered through 0.22 micron filter. |
|---|---|
| Storage: | Room Temp. |
| Synonym: | C₈H₁₈N₂O₄S (aq) |
| pH: | 7.50 ± 0.20 |
| QC Testing: | Appearance, pH, Conductivity, Osmolality, Low Endotoxin, DNase Free, RNase Free, Protease Free |
| Component Name | CAS | Molecular Weight | Concentration |
|---|---|---|---|
| HEPES | 7365-45-9 | 238.30 | 1 M |
| Sodium Hydroxide (Pellets) | 1310-73-2 | 40.00 | For pH Adjustment |
| Water for Injection (WFI) | 7732-18-5 | 18.02 | Rest |
Related Products
-
Tricine Buffer (0.2 M, pH 6.5)- BB-199Sku#: BB-199
-
MOPS Buffer (1 M, pH 8.0)- BBM-80Sku#: BBM-80
-
HEPES Buffer (1 M, pH 7.8)- BBH-78Sku#: BBH-78
-
HEPES Buffer (1 M, pH 8.5)- BBH-85Sku#: BBH-85
-
Tris-HCl Buffer (0.5 M, pH 6.8)- BBT-403Sku#: BBT-403