HBS-EP+ Buffer (1X, pH 7.4)
CAT#: HBS-110
- Useful in Surface Plasmon Resonance protocols
- Effective in maintaining pH and salt content while also performing as a wash buffer
- Ready-to-use solution, providing a buffering system within the pH range of 6.8-8.2
Available sizes:
Product Description
The HBS-EP+ Buffer (HEPES Buffered Saline with EDTA and Surfactant P20) is a variant of HEPES Buffered Saline, useful in SPR (Surface Plasmon Resonance) protocols. This reagent is formulated at a pH of 7.4 and can be used to prime in-unit pumps within SPR devices. Serving as both a running and binding buffer in such protocols, the HBS-EP+ solution is useful in assessing binding characteristic and biochemical dynamics between analytes.
Specifications
Method: | Filtered through 0.22-micron filter. |
---|---|
Storage: | Room Temp. |
Synonym: | HEPES Buffered Saline, SPR Running Buffer, Surface Plasmon Resonance Buffer |
pH: | 7.40 ± 0.20 |
QC Testing: | Appearance, pH |
Component Name | CAS | Molecular Weight | Concentration |
---|---|---|---|
HEPES | 7365-45-9 | 238.30 | 10 mM |
Sodium Chloride | 7647-14-5 | 58.44 | 150 mM |
EDTA Disodium Salt Dihydrate | 6381-92-6 | 372.24 | 3 mM |
Tween-20 | 7732-18-5 | ~1228 | 0.05% (v/v) |
Water | - | 18.02 | Rest |