HBS-EP Buffer (1X, pH 7.4)
CAT#: HBS-100
- Useful in Surface Plasmon Resonance protocols
- Effective in maintaining pH and salt content while also performing as a wash buffer
- Ready-to-use solution, providing a buffering system within the pH range of 6.8-8.2
Available sizes:
Product Description
The HBS-EP Buffer (HEPES Buffered Saline with EDTA and Surfactant P20) is a variant of HEPES Buffered Saline, useful in SPR (Surface Plasmon Resonance) protocols. This reagent is formulated at a pH of 7.4 and can be used to prime in-unit pumps within SPR devices. Serving as both a running and binding buffer in such protocols, the HBS-EP solution is useful in assessing binding characteristic and biochemical dynamics between analytes. This solution employs a lower concentration of Surfactant P20 than HBS-EP+ solutions.
Specifications
| Method: | Filtered through 0.22-micron filter. |
|---|---|
| Storage: | Room Temp. |
| Synonym: | HEPES Buffered Saline, SPR Running Buffer, Surface Plasmon Resonance Buffer |
| pH: | 7.40 ± 0.20 |
| QC Testing: | Appearance, pH |
| Component Name | CAS | Molecular Weight | Concentration |
|---|---|---|---|
| HEPES | 7365-45-9 | 238.30 | 10 mM |
| Sodium Chloride | 7647-14-5 | 58.44 | 150 mM |
| EDTA Disodium Salt Dihydrate | 6381-92-6 | 372.24 | 3 mM |
| Tween-20 | 9005-64-5 | ~1228 | 0.005% (v/v) |
| Water | 7732-18-5 | 18.02 | Rest |
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HBS-N Buffer (1X, pH 7.4)- HBS-130Sku#: HBS-130
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HBS-P+ Buffer (10X, pH 7.4)- HBS-121Sku#: HBS-121
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HBS-P+ Buffer (1X, pH 7.4)- HBS-120Sku#: HBS-120
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HBS-EP+ Buffer (1X, pH 7.4)- HBS-110Sku#: HBS-110
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HBS-EP+ Buffer (10X, pH 7.4)- HBS-111Sku#: HBS-111