Hank's Balanced Salt Solution, HBSS (10X, with Phenol Red)
CAT#: BSS-240
- Useful in cell and tissue culture and preservation
- Effective in providing cells with a stable pH and osmotic balance while mimicking physiological conditions
- Ready-to-use solution tested for pH
Available sizes:
Product Description
Hank’s Balanced Salt Solution (HBSS) with phenol red is a 10X isotonic solution that is useful in cell culture and tissue maintenance. It is composed of inorganic salts and a carbohydrate energy source, ensuring that cells are provided with the necessary nutrients while maintaining a stable pH and osmotic balance. Its balanced formulation is designed to preserve the structural and physiological integrity of cells while visually displaying any changes to pH, making it valuable for biological research and development.
Specifications
| Method: | Filtered through 0.22-micron filter. |
|---|---|
| Storage: | 2-8°C |
| Synonym: | HBSS |
| pH: | 7.40 ± 0.20 |
| QC Testing: | Appearance, pH |
| Notes: | Product comes with separate metal mix solution (Calcium Chloride Dihydrate [126 mM], Magnesium Chloride Hexahydrate [49 mM], Magnesium Sulfate [41 mM]) to avoid calcium phosphate precipitation. To prepare 1 L of 1X solution, add 100 mL of HBSS (10X), 10 mL of metal mix solution and 890 mL of deionized water. The pH of the 1X solution will be 7.4 ± 0.15. |
| Component Name | CAS | Molecular Weight | Concentration |
|---|---|---|---|
| Sodium Chloride | 7647-14-5 | 58.44 | 1.37 M |
| Potassium Chloride | 7447-40-7 | 74.55 | 53.3 mM |
| Potassium Phosphate Monobasic Anhydrous | 7778-77-0 | 136.09 | 4.4 mM |
| Sodium Phosphate Dibasic Anhydrous | 7558-79-4 | 141.96 | 3.4 mM |
| Sodium Bicarbonate | 144-55-8 | 84.01 | 41.7 mM |
| Dextrose | 50-99-7 | 180.16 | 55.6 mM |
| Phenol Red | 143-74-8 | 354.38 | 1 mM |
| Water | 7732-18-5 | 18.02 | Rest |
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