Hank's Balanced Salt Solution, HBSS (10X, with Phenol Red)

SKU#: BSS-240
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Product Description

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Method: Filtered through 0.22-micron filter.
Storage: 2-8°C
Synonym: HBSS
pH: 7.40 ± 0.20
QC Testing: Appearance, pH
Notes: Product comes with separate metal mix solution (Calcium Chloride Dihydrate [126 mM], Magnesium Chloride Hexahydrate [49 mM], Magnesium Sulfate [41 mM]) to avoid calcium phosphate precipitation. To prepare 1 L of 1X solution, add 100 mL of HBSS (10X), 10 mL of metal mix solution and 890 mL of deionized water. The pH of the 1X solution will be 7.4 ± 0.15.
Component NameCASMolecular WeightConcentration
Sodium Chloride 7647-14-5 58.44 1.37 M
Potassium Chloride 7447-40-7 74.55 53.3 mM
Potassium Phosphate Monobasic Anhydrous 7778-77-0 136.09 4.4 mM
Sodium Phosphate Dibasic Anhydrous 7558-79-4 141.96 3.4 mM
Sodium Bicarbonate 144-55-8 84.01 41.7 mM
Dextrose 50-99-7 180.16 55.6 mM
Phenol Red 143-74-8 354.38 1 mM
Water 7732-18-5 18.02 Rest

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