Glycine (100 mM)
CAT#: BPS-366
- For use in bioprocessing and biomolecule purification procedures
- Buffering agent capable of stabilizing proteins and enzymes, preserving target molecules
- Formulated with USP Compendial-Grade Raw Materials and tested for pH, Conductivity, Osmolality, Endotoxin, DNase, RNase, Protease
Available sizes:
Product Description
100mM Glycine is used in the affinity purification of antibodies, and within elution buffers. During affinity chromatography, an antibody bound to an antigen (or peptide) on a column can be detached by reducing the pH of the elution buffer. A glycine solution at pH 2.5-3.0 is commonly employed for this purpose because the low pH disrupts the antibody and antigen binding, allowing the antibody to be eluted from the column without denaturing it significantly. This solution is prepared in WFI-Quality Water.
Specifications
| Method: | Filtered through 0.22-micron filter. |
|---|---|
| Storage: | Room Temp. |
| pH: | 6.80 ± 0.20 |
| QC Testing: | Appearance, pH, Conductivity, Osmolality, Low Endotoxin, DNase Free, RNase Free, Protease Free |
| Component Name | CAS | Molecular Weight | Concentration |
|---|---|---|---|
| Glycine | 56-40-6 | 75.07 | 100 mM |
| Water for Injection (WFI) | 7732-18-5 | 18.02 | Rest |
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