CHAPS Lysis Buffer

CAT#: BP-114
  • Useful in cell lysis and extraction protocols
  • Supports solubilization and purification of membrane proteins under non-denaturing conditions
  • Ready-to-use solution tested for pH

Available sizes:

CAT#:
BP-177-500ml Before requesting a quote or making a purchase, please make sure you select the correct size.
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Product Description

The CHAPS lysis buffer, or 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate buffer, is a zwitterionic lysis buffer commonly used to lyse cells under nondenaturing conditions. This buffer supports applications including solubilization and purification of membrane proteins, and enzyme assays. Although CHAPS lysis buffer exhibits strong protein dissolution potentials, it still preserves protein conformation and protein-protein interaction for co-immunoprecipitation and pull down analyses.

Specifications

More Information
Method: Filtered through 0.22-micron filter.
Storage: 2-8°C
Synonym: CHAPS
pH: 7.40 ± 0.20
QC Testing: Appearance, pH
Notes: For long term storage, store product at -20°C.
Component NameCASMolecular WeightConcentration
Tris-Base / Tris-HCl 77-86-1 / 1185-53-1 121.14 / 157.60 50 mM
Sodium Chloride 7647-14-5 58.44 110 mM
EDTA 60-00-4 292.24 5 mM
CHAPS 75621-03-3 614.88 1% (w/v)
Water 7732-18-5 18.02 Rest

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