CHAPS Lysis Buffer (2X)
CAT#: BP-114X
- Useful in cell lysis and extraction protocols
- Supports solubilization and purification of membrane proteins under non-denaturing conditions
- Ready-to-use 2X solution tested for pH
Available sizes:
Product Description
The CHAPS lysis buffer, or 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate buffer, is a zwitterionic lysis buffer commonly used to lyse cells under nondenaturing conditions. This 2X buffer supports applications including solubilization and purification of membrane proteins, and enzyme assays. Although CHAPS lysis buffer exhibits strong protein dissolution potentials, it still preserves protein conformation and interaction for co-immunoprecipitation and pull down analyses.
Specifications
| Method: | Filtered through 0.22-micron filter. |
|---|---|
| Storage: | 2-8°C |
| Synonym: | CHAPS |
| pH: | 7.40 ± 0.20 (1X) |
| QC Testing: | Appearance, pH |
| Notes: | For long term storage, store product at -20°C. |
| Component Name | CAS | Molecular Weight | Concentration |
|---|---|---|---|
| Tris-Base / Tris-HCl | 77-86-1 / 1185-53-1 | 121.14 / 157.60 | 100 mM |
| Sodium Chloride | 7647-14-5 | 58.44 | 220 mM |
| EDTA (0.5 M, pH 7.4) | 60-00-4 | 292.24 | 10 mM |
| CHAPS | 75621-03-3 | 614.88 | 2% |
| Water | 7732-18-5 | 18.02 | Rest |
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CHAPS Lysis Buffer- BP-114Sku#: BP-114