Bicine Buffer (0.5 M, pH 8.0)
CAT#: BB-1062
- Useful in protein research, purification, biochemistry, and molecular biology
- Enhances protein stability and chromatography yield
- Ready-to-use solution, providing a buffering system within the pH range of 7.6-9.0
Available sizes:
Product Description
The Bicine Buffer is a zwitterionic buffer utilized to maintain pH within 7.5 to 9.0, making it suitable for processes that require a stable environment within this range. It is effective in several applications in molecular biology, including protein crystallization, enzymatic reactions, electrophoresis, and thin layer ion exchange chromatography. The pH of bicine buffer varies depending on the proportion of bicine to its conjugate base.
Specifications
| Method: | Filtered through 0.22-micron filter. |
|---|---|
| Storage: | 2-8°C |
| pH: | 8.00 ± 0.20 |
| QC Testing: | Appearance, pH |
| Component Name | CAS | Molecular Weight | Concentration |
|---|---|---|---|
| Bicine | 150-25-4 | 163.17 | 500 mM |
| Sodium Hydroxide | 1310-73-2 | 40 | For pH Adjustment |
| Water | 7732-18-5 | 18.02 | Rest |
Product Citations
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