Phospho-Protein Extraction Buffer
CAT#: BP-116P
- Useful in cell lysis and extraction protocols
- Effective in extraction and stability of target phosphorylated proteins
- Ready-to-use solution, providing a buffering system within the pH range of 7.0-8.0
Available sizes:
Product Description
The Phospho-Protein Extraction Buffer is a high-performance solution designed to extract and stabilize phosphorylated proteins from various biological samples. It lyses cell membranes and nuclear envelopes to release cytoplasmic and nuclear phospho-proteins, ensuring optimal conditions for phosphorylation assays. Ideal for research in signal transduction, cancer biology, and drug discovery, this buffer supports the study of protein interactions, phosphorylation events, and inhibitor screening.
Specifications
| Method: | Filtered through 0.22-micron filter. |
|---|---|
| Storage: | 2-8°C |
| Synonym: | Phosphoprotein Extraction Reagent, Phosphoprotein Lysis Buffer |
| pH: | 8.00 ± 0.15 |
| QC Testing: | Appearance, pH |
| Component Name | CAS | Molecular Weight | Concentration |
|---|---|---|---|
| Tris-Base / Tris-HCl | 77-86-1 / 1185-53-1 | 121.12 / 157.6 | 20 mM |
| Sodium Chloride | 7647-14-5 | 58.44 | 150 mM |
| Sodium Fluoride | 7681-49-4 | 41.99 | 100 mM |
| Nonidet P-40 Substitute | 9002-93-1 | ~680 | 1% (v/v) |
| Water | 7732-18-5 | 18.02 | Rest |
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