M9 Minimal Salts Solution
CAT#: BM-362
- Useful in non-selective isolation of E. Coli for molecular biology studies
- Effective in creating M9 minimal medium and for use in genetic experiments and protein expression
- Ready-to-use solution tested for Microbial Growth
Available sizes:
Product Description
M9 Minimal Salts solution contains buffering agents, a nitrogen source, ions such as phosphorus and sulfur, and metal ions. It is generally supplemented with glucose, amino acids, and vitamins for preparation of M9 minimal medium, which is used for non-selective cultivation of E. coli strains for cloning, production of DNA plasmids, and production of recombinant proteins. Upon addition of appropriate antibiotics, M9 minimal media can also be used for selective cultivation of E. coli strains
Specifications
| Method: | Filtered through 0.22-micron filter. |
|---|---|
| Storage: | 2-8°C |
| Synonym: | M9 Broth, M9 Medium, M9 Minimal |
| QC Testing: | Appearance, No Microbial Growth |
| Component Name | CAS | Molecular Weight | Concentration |
|---|---|---|---|
| Sodium Phosphate Dibasic Heptahydrate | 7782-85-6 | 268.07 | 1.28% (w/v) |
| Potassium Phosphate Monobasic Anhydrous | 7778-77-0 | 136.09 | 0.3% (w/v) |
| Sodium Chloride | 7647-14-5 | 58.44 | 0.05% (w/v) |
| Ammonium Chloride | 12125-02-9 | 53.49 | 0.1% (w/v) |
| Dextrose | 50-99-7 | 180.16 | 0.4% (w/v) |
| Magnesium Sulfate Anhydrous | 7487-88-9 | 246.5 | 2 mM |
| Calcium Chloride, Dihydrate | 10035-04-8 | 147.01 | 0.1 mM |
| Water | 7732-18-5 | 18.02 | Rest |
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