Krebs-Henseleit Buffer (with HEPES)
CAT#: BSS-265
- Useful in cell and tissue culture and preservation
- Effective in providing cells with a stable pH and osmotic balance
- Ready-to-use solution tested for pH
Available sizes:
Product Description
The Krebs-Henseleit buffer with HEPES is a balanced salt solution used in cell culture and tissue preservation to maintain physiological pH and osmotic balance, and is formulated at a pH of 7.40 ± 0.15. It contains glucose, magnesium sulfate, potassium phosphate, potassium chloride, sodium chloride, sodium bicarbonate, calcium chloride and HEPES. This buffer is ideal for experiments requiring stable pH, such as calcium imaging and tissue preservation.
Specifications
| Method: | Filtered through 0.22-micron filter. |
|---|---|
| Storage: | 2-8°C |
| Synonym: | Krebs-Henseleit Solution |
| pH: | 7.40 ± 0.15 |
| QC Testing: | Appearance, pH |
| Notes: | This product was formerly listed as PY-946. |
| Component Name | CAS | Molecular Weight | Concentration |
|---|---|---|---|
| HEPES | 7365-45-9 | 238.3 | 10 mM |
| Potassium Chloride | 7447-40-7 | 74.55 | 4.7 mM |
| Magnesium Sulfate Anhydrous | 7487-88-9 | 120.37 | 1.2 mM |
| Potassium Phosphate Monobasic Anhydrous | 7778-77-0 | 136.09 | 1.2 mM |
| Sodium Bicarbonate | 144-55-8 | 84.01 | 25 mM |
| Dextrose | 50-99-7 | 180.16 | 11.1 mM |
| Calcium Chloride Dihydrate | 10035-04-8 | 147.01 | 1.25 mM |
| Sodium Chloride | 7647-14-5 | 58.44 | 118 mM |
| Water | 7732-18-5 | 18.02 | Rest |
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