Glycine Solution (2.5M, Low Endotoxin)
CAT#: BB-4375
- Useful in cell culture, protein research, and biochemistry
- Buffering agent capable of stabilizing proteins and enzymes, preserving target molecules
- Ready-to-use 2.5M solution, providing a buffering system within the pH range of 2.2-3.6
Available sizes:
Product Description
2.5 M glycine is used in the affinity purification of antibodies, particularly in elution buffers. During affinity chromatography, an antibody bound to an antigen (or peptide) on a column can be detached by reducing the pH of the elution buffer. A glycine solution at pH 2.5-3.0 is commonly employed for this purpose because the low pH disrupts the antibody and antigen binding, allowing the antibody to be eluted from the column without denaturing it significantly.
Specifications
| Method: | Filtered through 0.22-micron filter. |
|---|---|
| Storage: | Room Temp. |
| Synonym: | Câ‚‚Hâ‚…NOâ‚‚ (aq), Glycine Buffer |
| pH: | 7.40 ± 0.50 |
| QC Testing: | Appearance |
| Component Name | CAS | Molecular Weight | Concentration |
|---|---|---|---|
| Glycine | 56-40-6 | 75.07 | 2.5 M |
| Water | 7732-18-5 | 18.02 | Rest |
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