Tris-Buffered Saline (TBS, 10X, pH 7.4)

COMPOSITION: 250 mM Tris, 27 mM KCl, and 1.37 M NaCl, pH 7.4 ± 0.2.

METHOD: Prepared in 18.2 megohms water and filtered through 1-micron filter.

PH: The pH of the 1X should be 7.4 ± 0.2.

NOTES: To make 1X, dilute 10-fold with DI water

STORAGE: Store at room temperature.