Thrombin Buffer (His-tag protein purification from mammalian cells)

COMPOSITION: 50 mM Tris-HCl (pH 8.0), 150 mM NaCl, 2 mM CaCl2.
METHOD: Prepared in 18.2 megohms water and filtered through 1 micron filter.
STORAGE: Store at 2-8 degree Celsius.

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