TBST (10X, with 0.5% Tween-20, pH 7.6)

COMPOSITION: 500 mM Tris, 1.5 M NaCl and 0.5% Tween-20

METHOD: Prepared in 18.2 megohms water and autoclaved at 15 psi for 30 min on liquid cycle.

PH: pH 7.6 ± 0.2.

NOTES: To make 1X, dilute 10-fold with DI water.

STORAGE: Store at room temperature.