Phospho-Protein Extraction Buffer III

COMPOSITION: 20 mM Tris-HCl (pH 7.5), 150 mM NaCl, 3 mM NaF, 0.2% NP-40),1 mM EDTA, 1 mM Beta-glycerophosphate and 10% Glycerol.
METHOD: Prepared in 18.2 megohms water and filtered through 1-micron filter.
STORAGE: Store at 2-8 degree Celsius.

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